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Antibodies bound to the blocking peptide not bind to your epitope to the target protein. By comparing the staining within the blocked antibody versus the antibody alone, one can see which staining is certain.

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This guide outlines even further feasible optimization of cellular and immunoassay parameters to be certain the absolute best results are...

This guide outlines further achievable optimization of mobile and immunoassay parameters to make sure the best possible outcomes are...

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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the photographs or hyperlinks will redirect you to a web site hosted by BenchSci that gives 3rd-bash scientific content. Neither the content material nor the BenchSci engineering and procedures for choice are already evaluated by us; we have been providing them as-is and without guarantee of any type, including for use or software in the Thermo Fisher Scientific goods offered.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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Blocking peptides are peptides that bind especially to your concentrate on antibody and block antibody binding. These peptide commonly is made up of the epitope identified from the antibody.

Target Information and facts Cyclin E belongs into the remarkably conserved cyclin family members, whose associates are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation patterns which contribute to your temporal coordination of every mitotic celebration. Cyclin E kinds a fancy with and functions as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates within the G1-S section boundary and is particularly degraded as cells progress through S phase.

Overexpression of this gene has become noticed in many tumors, which ends up in chromosome instability, and so may perhaps add to tumorigenesis. This protein was identified to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a crucial function in selling cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two supplemental splice variants had been noted but in depth nucleotide sequence facts just isn't yet accessible.

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